Nucleotide sequence encoding the membrane protein of the IBV strain 6/82

Sequence and Phylogenetic Analysis of Membrane (M) Gene of Infectious Bronchitis Viruses Isolated in Iran during 2014 - 2015

Background and Aims: Avian infectious bronchitis virus (IBV) has a worldwide distribution and mutations occurring in the large viral genome of IBV have led to extensive antigenic variations among IBVs. This is the first study conducted to determine the complete membrane (M) gene sequences of different Iranian IBV genotypes. Materials and Methods: The M gene of three 793/B (IBKG1,6,7), one Mass...

Nucleotide Sequence of Gene Encoding Capsid Protein VPI of Foot-and-Mouth Disease Virus / Type O1 Iran

Cloning and Sequence Analysis of Gene Encoding OipA from Iranian Clinical Helicobacter pylori

Background: Outer inflammatory protein A (OipA) is one of the important adhesins of H. pylori and a valuable candidate for vaccine development. Its gene is under "on-off" switch status which correlates with OipA protein expression. Objectives: We aimed to obtain a recombinant OipA clone (with "on" status) from an Iranian clinical isolate. Materials and Methods: A clinical H. pylori-isolate demo...

Antifungal Activity of Heterologous Expressed Chitinase 42 (Chit42) from Trichoderma atroviride PTCC5220

The cDNA from the mycoparasitic fungus Trichoderma atroviride PTCC5220 encoding a 42 kDa chitinase (Chit42) was isolated. The nucleotide sequence of the cDNA fragment as having a 1263 bp open reading frame that encodes a 421 amino acid polypeptide, and a high homology was found withother reported Chit42 belonging to the Trichoderma sp. The 22 amino acid N-terminal sequence is a putative s...

Molecular Cloning and Expression of Nucleocapsid Gene of Chicken Infectious Bronchitis Virus Strain Massachusetts H120

Chicken infectious bronchitis virus (IBV) causes severe respiratory and renal dysfunction in chickens. In the present study, we used reverse transcription-polymerase chain reaction (RT-PCR) to amplify the nucleocapsid (N) protein gene of strain Massachusetts (Mass) H120 of IBV that is commonly used in the vaccine production in Iran. The PCR product with the expected size of 1.2 kb was cloned in...